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BCR::ABL1 V299L dasatinib-resistance mutation

Deterministic view of the source YAML entity. Clinical authority remains with the cited source IDs and reviewer sign-off state.

IDBIO-BCR-ABL1-V299L
TypeBiomarker
Aliases
ABL1 p.V299LBCR-ABL V299LBCR-ABL1 V299L resistance mutationBCR::ABL1 V299L — мутація резистентності до дазатинібу
Statusreviewed 2026-04-27 | pending_clinical_signoff
DiseasesNone declared
SourcesSRC-ELN-CML-2020 SRC-ELN-CML-2025 SRC-ESMO-CML-2017

Biomarker Facts

Biomarker typegene_mutation
Mutation details{"exon": "5", "functional_impact": "dasatinib and bosutinib resistance; nilotinib and ponatinib retain activity", "gene": "ABL1", "hgvs_protein": "p.V299L", "variant_type": "missense"}
Measurement
MethodBCR::ABL1 kinase-domain Sanger sequencing OR targeted NGS at TKI failure
Unitscategorical (positive | negative); VAF % when NGS
Actionability lookup{"gene": "ABL1", "variant": "V299L"}
Related biomarkersBIO-BCR-ABL1 BIO-BCR-ABL1-T315I BIO-BCR-ABL1-F317L

Notes

Resistance to BOTH dasatinib and bosutinib via P-loop conformational change. IC50 for dasatinib elevated ~15-20x; bosutinib ~25-50x. Nilotinib retains activity (IC50 unchanged). Ponatinib and asciminib both active. Per ELN-2020 / ELN-2025: detection on dasatinib or bosutinib failure → switch to nilotinib (preferred 2G-TKI option) or ponatinib. Distinguishing V299L from F317L is clinically meaningful: F317L spares bosutinib whereas V299L does not.

Used By

Biomarker